打开file.txt并找到其基因可能的起始和终止位置

Hi I have a file and I would like to open it and find the start and end positions of its genes,also I have some extra imformations.The beginning of each gene is mapped by the following pattern. There is an 8 letter consensus known as the Shine-Dalgarno sequence (TAAGGAGG) followed by 4-10 bases downstream before the initiation codon (ATG). However there are variants of the Shine-Dalgarno sequence with the most common of which being [TA][AC]AGGA[GA][GA].The end of the gene is specified by the stop codon TAA, TAG and TGA. It must be taken care the stop codon is found after the correct Open.Reading Frame (ORF). Now I have make a txt file with genome and I open it with this code,and the error begin when I go to read the genome and put start and end.Any help?Thanks a lot.: Hi I have a file and I would like to open it an