VS ASP.NET 500服务器错误

Hey guys, I'm having a super weird problem with my VS 2008 solution. We had this hand-coded ASP.NET compiled web app on our old IIS6/Win2003 server, working great, moved it to our new IIS7/Win2008 server, still working great, but when I try to compile the application and publish it again to our new Win2008 server, I get server 500 errors. It's ASP.NET 2.0 with AJAX extensions and AJAX control toolkit.Hey guys, I'm having a super weird problem with