ASP.Net MVC IIS6和ACL错误

I have an asp.net mvc 3 app installed on IIS6 and I am getting ACL errors (401.3 errors) when trying to access it. It is running as a virtual app under the default web. I have gone through what I believe are the correct security setting on the respective folders. I have an asp.net mvc 3 app installed on IIS6 a