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How to determine the signal effect of something in certain process?

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How to prove the function of chemicals as a signal in physiological process ?  It is a very common question in research of biology,  especial for molecular botany, rather than ecology. However, the logical and careful experiment design is necessary in science study. I like science for it is not expressive skill or  word play.   Today, I read a  traditional article on plant physiology, the same as the name of the published journal.  It is about common reed that I am interested in and have done some works on, so I found it. How to prove the function of chemicals as a



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